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KMID : 0385520200330010001
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Analytical Science & Technology
2020 Volume.33 No. 1 p.1 ~ p.10
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Enantiomeric purity test of R-(+)-alpha lipoic acid by HPLC using immobilized amylose-based chiral stationary phase
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Le Thi-Anh-Tuyet
Pham Thuy-Vy
Mai Xuan-Lan
Song Chai-Lin
Woo Sung-Jun
Jeong Cheol-Hee
Choi Sung-Youn
Phan Thanh Dung
Kim Kyeong-Ho
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Abstract
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Alpha lipoic acid, an antioxidant, is widely used for treatment of various diseases. It is a racemic mixture, with R-(+)-α lipoic acid exhibiting greater potency, bioavailability, and effectiveness than those of the S-form. Thus, selective R-(+)-α lipoic acid has been recently used in various applications, necessitating the development of a method to test the enantiomeric impurity in R-(+)-α lipoic acid. We developed a simple and fast high-performance liquid chromatography method using a new immobilized amylose-based chiral column (Chiralpak IA-3). Design of experiment was applied to accurately predict the effects and interactions among various factors affecting the analytical parameters and to optimize the chromatographic conditions. This optimized method could completely separate the two enantiomer peaks with a resolution > 1.8 within a short running time (9 min). Then, the optimized method was validated according to the guidelines of the International Conference on Harmonization and applied for quantification of S-(?)-α lipoic acid in some commercial R-(+)- α lipoic acid tromethamine raw material. Our results suggested that the developed method could be used for routine quality control of R-(+)-α lipoic acid products.
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KEYWORD
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R-(+)-α lipoic acid, High-performance liquid chromatography (HPLC), Enantiomeric purity test, Immobilized chiral stationary phase
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